Sodium Dodecyl Sulfate-Polyacrylamide Solution-Filled Capillary Electrophoresis of Proteins Using Stable Linear Polyacrylamide-Coated Capillary.

Capillary electrophoresis/tandem mass spectrometry for analysis of proteins from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip Tip is used before CE/TOFMS analysis which allows for detection of protein spots with <1 picomol...

High-temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis.

The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...

Improvement of a capillary electrophoresis-chemiluminescence detection system for using a polyacrylamide-coated capillary.

Rapid sodium dodecyl sulfat/polyacrylamide gel electrophoresis of urinary proteins.

We describe a procedure for the convenient separation of proteins by sodium dodecyl sulfate/polyacrylamide gel electrophoresis of urine from cases of renal disease. A precipitation method that requires no special apparatus was used to concentrate the urinary proteins; for electrophoretic separation we used a commercially supplied polyacrylamide/cellulose gel slab. This method seems to be valuab...

Polyacrylamide gel electrophoresis of mycoplasma proteins in sodium dodecyl sulphate.

Electrophoresis of constituent proteins in polyacrylamide gels has become a useful aid in the identification of mycoplasmas. Razin & Rottem (1967) showed that mycoplasma proteins dissolved in phenol and acetic acid give reproducible, species-specific electrophoretic patterns when separated in the presence of acetic acid and urea. We encountered difficulties when using this technique. Mycoplasma...